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ImageQuant TL 7.0 User Manual 28-9175-41 Edition AA 9 1 Introduction 1.1 Welcome to ImageQuant TL The ImageQuantâ„¢ TL software provides a complete software package to meet your image analysis requirements. The software consists of the 1D, Array, Colony Counter and Toolbox modules integrated into one package, with each. Imagequant Tl Iqtl Software Ge Healthcare Life Nik Silver Efex Pro 2 Presets Downloaded Star Wars Battlefront 2 Torrent Tpb New Order Remastered Rar Install Adobe Flash Plugin Puppy Linux Download Burrows Casebook On Contract 3rd Edition Mock Trial Opening Statements For The Prosecutor Soundboard.
Ready for use in less than five minutesImageQuant LAS 500 imager consists of a touchscreen, sample chamber, USB port, camera and light sources. The 8.3-megapixel CCD camera is ready for use in less than five minutes. Reliable Peltier cooling combined with binning significantly reduces noise levels and improves both the sensitivity and linearity of the system. Flexible imaging modes for Optimal chemiluminescence detectionSignal intensity can be detected either automatically or semi-automatically by specifying an area of interest. In addition, single (manual) or multiple (incremental) exposures can be set by the operator.
The camera is designed for optimal chemiluminescence detection with regard to sensitivity and dynamic range. Three LED light sourcesThe system features three LED light sources: a combined blue epi-/UV epi-light and a white epi-light. The combined blue epi-/UV epi-light allows imaging of fluorescence signals above 550 nm. This feature makes the camera well suited for DNA stain imaging with SYBRâ„¢ Green, ethidium bromide, or Deep Purple Total Protein Stain. A fast and easy way to verify the molecular weight of detected protein bandsThe white epi-light source (470 to 635 nm) enables documentation of colorimetric markers and stains, such as Coomassie Blue or Silver. Moreover, the white light mode can be combined with the chemiluminescence mode to automatically generate an overlay image of the colored marker and chemiluminescent sample images. This provides a fast and easy way to verify the molecular weight of detected bands, and identification of band of interest if multiple bands are generated.
SoftwareIn addition to the on-board software, images acquired using ImageQuant LAS 500 can be analyzed using ImageQuant TL (IQTL). IQTL is an automated and easy-to-use general-purpose gel, blot, and array image analysis software. IQTL requires installation on an external computer and cannot be installed on ImageQuant LAS 500.
Western blot detectionWestern blotting, also known as immunoblotting, or protein blotting, is a well-established and widely used technique for the detection and analysis of proteins. A variety of detection systems, based on chemiluminescence, chemifluorescence, fluorescence, chromogenic, or radioisotopic detection are available. Chemiluminescence is reliable and well known for having the greatest sensitivity of any available detection method.Further, there has been a decline in usage of radioisotopic and chromogenic reagents due to safety issues with handling radioactive isotopes and poor sensitivity with chromogenic reagents, which has meant, enzyme-based chemiluminescent and direct fluorescence have been extensively developed and are now the preferred method for detection due to their improved sensitivity and wider dynamic range. What is chemiluminescence western blot detection?Chemiluminescence Western Blot detection is based on antibodies conjugated to horseradish peroxidase (HRP) that catalyze the oxidation of luminol in presence of peroxide, resulting in light emission. HRP has several advantages over other enzymes such as alkaline phosphatase (AP). HRP can be easily conjugated to antibodies or streptavidin (which binds with extraordinarily high affinity to biotin, a commonly used tag, and can be used with different chemiluminescent reagents.
Considerable efforts have been made to develop HRP-based detection reagents to obtain higher detection sensitivity, stronger light intensity and long-lasting signals.
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